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cck 8 reagent  (Dojindo Labs)


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    Dojindo Labs cck 8 reagent
    Cck 8 Reagent, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 60332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cck+8+reagent/Cell+Counting+Kit-8/pmc13634581-387-5-7
    Average 99 stars, based on 60332 article reviews
    cck 8 reagent - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: .. CCK-8 reagent (10 μL, DOJINDO, Kumamoto, Japan) was added to each well at 0, 24, 48, and 72 h after transfection and incubated at 37 °C for 4 h. Absorbance at 450 nm was measured using a microplate reader (PerkinElmer EnVision, Massachusetts, USA). .. CRC cells (2 × 10 4 cells/well) were seeded onto Transwell inserts (8 μm, Corning, New York, USA) coated or uncoated with Matrigel.

    Article Title: PDZK1 is downregulated in ovariectomized mice with angiotensin II-induced hypertension and interacts with β2-AR to regulate endothelial ERK1/2/c-Fos signaling
    Article Snippet: .. CCK-8 reagent (Dojindo, Kumamoto, Japan) was added according to the manufacturer’s instructions, and absorbance was measured at 450 nm using an EnSpire microplate reader (PerkinElmer, Waltham, MA, USA). ..

    Article Title: FABP7 Enhances Endometrial Cancer Cell Migration and Stemness by Activating the Wnt/β-catenin Pathway.
    Article Snippet: Endometrial cancer is a common malignancy of the female reproductive system and poses a significant threat to women’s health.. Fatty acid binding protein 7 (FABP7) plays a key role in regulating lipid metabolism, signal transduction, and gene expression, and has been implicated in the progression of various cancers.. However, its role in endometrial cancer remains unclear.

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1
    Article Snippet: .. Briefly, cells were seeded into 96-well plates and cultured for 0, 24, 48, and 72 h. CCK-8 reagent (Dojindo, CK04) was added to each well and incubated according to the manufacturer’s protocol. ..

    Article Title: Salvianolic acid B suppresses non-small cell lung cancer growth by promoting Nrf2 proteasomal degradation and triggering autophagy-dependent ferroptosis.
    Article Snippet: Non-small cell lung cancer (NSCLC) constitutes the predominant lung cancer subtype and remains a leading cause of cancerrelated mortality worldwide, underscoring an imperative need for mechanistically informed therapeutic strategies.. Salvianolic acid B (SalB), a bioactive polyphenolic compound isolated from Salvia miltiorrhiza, has demonstrated antitumor potential across several malignancies; however, the precise molecular mechanisms governing its activity in NSCLC remain largely undefined.. The anticancer activity of SalB was evaluated in human NSCLC cell lines (A549, H460 and PC-9) and in an A549 xenograft mouse model. Apoptosis, proliferation, autophagy, oxidative stress, ferroptosis-associated phenotypes, and mitochondrial function were assessed using flow cytometry, clonogenic assays, immunofluorescence, transmission electron microscopy, and Western blotting.

    Article Title: Multi-omics analysis of LFA-1 peptide-functionalized silk fibroin nanofibers targeting RPE and activating AMPK–HO-1 signaling to suppress pyroptosis and alleviate diabetic retinopathy progression
    Article Snippet: Diabetic retinopathy (DR) is one of the leading causes of adult blindness, and pyroptosis of retinal pigment epithelial (RPE) cells plays a key role in its pathogenesis.. To achieve precise delivery of the multiple active components of the traditional Chinese medicine formula Zishen Jianpi Huayu Pills (ZSJP) to DR lesions, we constructed an LFA-1 peptide-modified silk fibroin nanofiber system (PCL/SF-L@ZSJP) that targets and activates the AMPK/HO-1 signaling pathway in RPE cells.. Nanofibers were prepared by coaxial electrospinning and systematically characterized.

    Article Title: Synchronized osteochondral regeneration using a dynamic sacrificial‑phase injectable macroporous PEO‑gelatin‑GelMA hydrogel delivering BMP‑14‑engineered BMSCs
    Article Snippet: .. On days 1, 3, and 5, 10 μL of CCK− 8 reagent (Dojindo, Kumamoto, Japan) was added to each well and incubated for 2 h at 37◦C in the dark. ..

    Transfection:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: .. CCK-8 reagent (10 μL, DOJINDO, Kumamoto, Japan) was added to each well at 0, 24, 48, and 72 h after transfection and incubated at 37 °C for 4 h. Absorbance at 450 nm was measured using a microplate reader (PerkinElmer EnVision, Massachusetts, USA). .. CRC cells (2 × 10 4 cells/well) were seeded onto Transwell inserts (8 μm, Corning, New York, USA) coated or uncoated with Matrigel.

    Incubation:

    Article Title: Identification of PLXNC1 as a novel biomarker for consensus molecular subtype 4 in colorectal cancer
    Article Snippet: .. CCK-8 reagent (10 μL, DOJINDO, Kumamoto, Japan) was added to each well at 0, 24, 48, and 72 h after transfection and incubated at 37 °C for 4 h. Absorbance at 450 nm was measured using a microplate reader (PerkinElmer EnVision, Massachusetts, USA). .. CRC cells (2 × 10 4 cells/well) were seeded onto Transwell inserts (8 μm, Corning, New York, USA) coated or uncoated with Matrigel.

    Article Title: FABP7 Enhances Endometrial Cancer Cell Migration and Stemness by Activating the Wnt/β-catenin Pathway.
    Article Snippet: Endometrial cancer is a common malignancy of the female reproductive system and poses a significant threat to women’s health.. Fatty acid binding protein 7 (FABP7) plays a key role in regulating lipid metabolism, signal transduction, and gene expression, and has been implicated in the progression of various cancers.. However, its role in endometrial cancer remains unclear.

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1
    Article Snippet: .. Briefly, cells were seeded into 96-well plates and cultured for 0, 24, 48, and 72 h. CCK-8 reagent (Dojindo, CK04) was added to each well and incubated according to the manufacturer’s protocol. ..

    Article Title: Early‐Life Manganese Overexposure Activates Microglial Phagocytosis through the YY1/TREM2 Axis Leading to Hippocampal Synaptic Remodeling in Mice
    Article Snippet: .. Following exposure, 10 μL of CCK‐8 reagent (Dojindo, Japan) was added to each well and incubated for 1 h at 37°C under standard culture conditions. ..

    Article Title: Salvianolic acid B suppresses non-small cell lung cancer growth by promoting Nrf2 proteasomal degradation and triggering autophagy-dependent ferroptosis.
    Article Snippet: Non-small cell lung cancer (NSCLC) constitutes the predominant lung cancer subtype and remains a leading cause of cancerrelated mortality worldwide, underscoring an imperative need for mechanistically informed therapeutic strategies.. Salvianolic acid B (SalB), a bioactive polyphenolic compound isolated from Salvia miltiorrhiza, has demonstrated antitumor potential across several malignancies; however, the precise molecular mechanisms governing its activity in NSCLC remain largely undefined.. The anticancer activity of SalB was evaluated in human NSCLC cell lines (A549, H460 and PC-9) and in an A549 xenograft mouse model. Apoptosis, proliferation, autophagy, oxidative stress, ferroptosis-associated phenotypes, and mitochondrial function were assessed using flow cytometry, clonogenic assays, immunofluorescence, transmission electron microscopy, and Western blotting.

    Article Title: Multi-omics analysis of LFA-1 peptide-functionalized silk fibroin nanofibers targeting RPE and activating AMPK–HO-1 signaling to suppress pyroptosis and alleviate diabetic retinopathy progression
    Article Snippet: Diabetic retinopathy (DR) is one of the leading causes of adult blindness, and pyroptosis of retinal pigment epithelial (RPE) cells plays a key role in its pathogenesis.. To achieve precise delivery of the multiple active components of the traditional Chinese medicine formula Zishen Jianpi Huayu Pills (ZSJP) to DR lesions, we constructed an LFA-1 peptide-modified silk fibroin nanofiber system (PCL/SF-L@ZSJP) that targets and activates the AMPK/HO-1 signaling pathway in RPE cells.. Nanofibers were prepared by coaxial electrospinning and systematically characterized.

    Article Title: Synchronized osteochondral regeneration using a dynamic sacrificial‑phase injectable macroporous PEO‑gelatin‑GelMA hydrogel delivering BMP‑14‑engineered BMSCs
    Article Snippet: .. On days 1, 3, and 5, 10 μL of CCK− 8 reagent (Dojindo, Kumamoto, Japan) was added to each well and incubated for 2 h at 37◦C in the dark. ..

    96 Well Plates:

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1
    Article Snippet: .. Briefly, cells were seeded into 96-well plates and cultured for 0, 24, 48, and 72 h. CCK-8 reagent (Dojindo, CK04) was added to each well and incubated according to the manufacturer’s protocol. ..

    Article Title: Multi-omics analysis of LFA-1 peptide-functionalized silk fibroin nanofibers targeting RPE and activating AMPK–HO-1 signaling to suppress pyroptosis and alleviate diabetic retinopathy progression
    Article Snippet: Diabetic retinopathy (DR) is one of the leading causes of adult blindness, and pyroptosis of retinal pigment epithelial (RPE) cells plays a key role in its pathogenesis.. To achieve precise delivery of the multiple active components of the traditional Chinese medicine formula Zishen Jianpi Huayu Pills (ZSJP) to DR lesions, we constructed an LFA-1 peptide-modified silk fibroin nanofiber system (PCL/SF-L@ZSJP) that targets and activates the AMPK/HO-1 signaling pathway in RPE cells.. Nanofibers were prepared by coaxial electrospinning and systematically characterized.

    Cell Culture:

    Article Title: A palmitoylation-associated three-gene signature predicts prognosis, immune features, and drug sensitivity in gastric cancer: functional validation of CTHRC1
    Article Snippet: .. Briefly, cells were seeded into 96-well plates and cultured for 0, 24, 48, and 72 h. CCK-8 reagent (Dojindo, CK04) was added to each well and incubated according to the manufacturer’s protocol. ..



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    ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. <t>(C)</t> <t>CCK‐8</t> cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.
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    ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. <t>(C)</t> <t>CCK‐8</t> cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.
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    ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. <t>(C)</t> <t>CCK‐8</t> cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.
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    Image Search Results


    ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. (C) CCK‐8 cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.

    Journal: Cancer Reports

    Article Title: Integrated Transcriptomic Analysis Identifies ENO3 as a Candidate Lactate Metabolism‐Associated Biomarker in Colorectal Cancer Metastasis

    doi: 10.1002/cnr2.70723

    Figure Lengend Snippet: ENO3 is associated with CRC prognosis and contributes to tumor‐cell phenotypes. (A) Violin plot showing the differential expression of ENO3 in CRC tumor tissues compared to adjacent normal tissues, utilizing data from the TCGA COADREAD cohort. Statistical significance was determined by a two‐tailed Wilcoxon rank‐sum test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (B) Kaplan–Meier survival curve illustrating the OS of CRC patients stratified by high and low ENO3 expression levels (grouped by the median expression value). The p ‐value was calculated using the log‐rank test. (C) CCK‐8 cell proliferation curve comparing the viability of MC38 wild‐type (WT) and ENO3 knockout (KO) cells over a period of 96 h. Data are presented as mean ± SD from n = 3 biological replicates. Statistical analysis was performed using two‐way ANOVA with Sidak's multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. (D) Hematoxylin and eosin (H&E) staining of lung tissues from NSG mice inoculated by tail‐vein injection with MC38 WT or ENO3‐KO cells, showing pulmonary lesions in the experimental lung colonization model. Representative images are shown at ×100 magnification. Scale bar, 100 μm. (E) Quantification of pulmonary tumor nodules in NSG mice inoculated with MC38 WT or ENO3‐KO cells. Data are presented as mean ± SD. Each dot represents an individual mouse ( n = 5 mice per group). Statistical significance was determined using a two‐tailed unpaired t ‐test.

    Article Snippet: Proliferation rates were quantified using CCK‐8 reagent (Vazyme #A311‐01).

    Techniques: Quantitative Proteomics, Two Tailed Test, Expressing, CCK-8 Assay, Knock-Out, Analysis, Staining, Injection